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Briefly, cumulus-free oocytes were incubated in M199 containing 0.1% PVA and 500 nM Mitotracker Red for 30 min at 38.5°C in an atmosphere of 5% CO 2 and saturated humidity
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Perhaps Craigslist’s single greatest cultural contribution, and my favorite place to lurk, is the “missed connections.” The feature has inspired countless copycats, artistic reinterpretations, human interest stories, and analyses (one in particular extrapolated that Monday evenings are the most lovelorn time across the country)
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